COUP TF1 Recombinant Rabbit Monoclonal Antibody [JE31-95] (HA721543)
Catalog# HA721543
Rabbit Monoclonal to COUP TF1
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of COUP TF1 on different lysates with Rabbit anti-COUP TF1 antibody (HA721543) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 8 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721543) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 8 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721543) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.