COX IV Recombinant Rabbit Monoclonal Antibody [JJ09-05] (ET1701-63)
Catalog# ET1701-63
Rabbit Monoclonal to COX IV
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
FC
-
Human
-
Mouse
-
Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: Mouse heart tissue lysate (30 µg/Lane)
Lane 4: Rat heart tissue lysate (30 µg/Lane)
Predicted band size: 20 kDa
Observed band size: 15 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
COX IV Recombinant Rabbit Monoclonal Antibody [JJ09-05] (ET1701-63)
Immunogen
Synthetic peptide within human cox iv aa 21-70 / 169.
Host
Rabbit
Positive Control
HeLa cell lysate, HepG2 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, HepG2, MCF-7, human liver tissue, human liver carcinoma tissue, human colon carcinoma tissue, human kidney tissue, mouse colon tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ09-05
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:200
-
IHC-P
-
1:400-1:1,000
-
FC
-
1:50-1:100
-
IP
-
Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 20 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
COX IV
SYNONYMS
AL024441 antibody;COX 4 antibody;COX IV 1 antibody;COX IV antibody;COX IV-1 antibody;Cox4 antibody;COX41_HUMAN antibody;Cox4a antibody;COX4B antibody;COX4I1 antibody;COX4I2 antibody;COX4L2 antibody;COXIV antibody;Cytochrome c oxidase polypeptide IV antibody;Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody;Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody;Cytochrome C Oxidase subunit IV antibody;Cytochrome c oxidase subunit IV isoform 1 antibody;Cytochrome c oxidase subunit IV isoform 2 (lung) antibody;Cytochrome c oxydase subunit 4 antibody;dJ857M17.2 antibody;MGC105470 antibody;MGC72016 antibody
SEQUENCE SIMILARITIES
Belongs to the cytochrome c oxidase IV family.
TISSUE SPECIFICITY
Ubiquitous.
SUBCELLULAR LOCATION
Mitochondrion inner membrane.
FUNCTION
Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.
CITATIONS
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.