CPT2 Recombinant Rabbit Monoclonal Antibody [SN06-70] (ET1611-64)
Catalog# ET1611-64
Rabbit Monoclonal to CPT2
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: PC-12 cell lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat kidney tissue lysate
Cell lysates/proteins at 20 µg/Lane.
Tisssue lysates/proteins at 40 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 67 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CPT2 Recombinant Rabbit Monoclonal Antibody [SN06-70] (ET1611-64)
Immunogen
Synthetic peptide within human cpt2 aa 609-658 / 658.
Host
Rabbit
Positive Control
Hela cell lysate, 293 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, SK-Br-3, SW480, human liver carcinoma tissue, human kidney tissue, mouse kidney tissue, human muscle tissue, human colon carcinoma tissue, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN06-70
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:1,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 74 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CPT2
SYNONYMS
Carnitine O palmitoyltransferase 2 antibody;Carnitine O palmitoyltransferase 2 mitochondrial antibody;Carnitine O-palmitoyltransferase 2 antibody;Carnitine palmitoyltransferase 2 antibody;Carnitine palmitoyltransferase II antibody;CPT 1 antibody;CPT 2 antibody;CPT II antibody;CPT1 antibody;CPT2 antibody;CPT2_HUMAN antibody;CPTASE antibody;CPTII antibody;IIAE4 antibody;mitochondrial antibody
SEQUENCE SIMILARITIES
Belongs to the carnitine/choline acetyltransferase family.
SUBCELLULAR LOCATION
Mitochondrion inner membrane, nucleolus, nucleoplasm.
FUNCTION
The protein encoded by this gene is a nuclear protein which is transported to the mitochondrial inner membrane. Together with carnitine palmitoyltransferase I, the encoded protein oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders. Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites. Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters.
CITATIONS
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Xu Xinyue,et al
Activation of LONP1 by 84-B10 alleviates aristolochic acid nephropathy via re-establishing mitochondrial and peroxisomal homeostasis
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WB
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Mouse
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Citation
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Quercetin Ameliorates Fatty Acid Oxidation in the Pathogenesis of NAFLD Based on Network Pharmacology and Molecular Docking
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WB
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Rat
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Citation
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