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CRISPR-Cas9 Rabbit Polyclonal Antibody (R1403-1)

Price: Regular price $315.00

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Catalog# R1403-1

Rabbit Polyclonal to CRISPR-Cas9

Tested for

  • WB

  • ELISA

Reacts with

  • Staphylococcus aureus

Specifications

Product Type

Rabbit polyclonal primary

Product Name

CRISPR-Cas9 Rabbit Polyclonal Antibody (R1403-1)

Immunogen

Synthetic peptide within streptococcusthermophilus crispr-cas9 aa 374-415/1388.

Host

Rabbit

Positive Control

Cas9 transfected 293 cells

Conjugation

Unconjugated

Clonality

Polyclonal

RRID

AB_3073290

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ELISA

  • 1:10,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Immunogen affinity purified.

Isotype

IgG

TARGET

UNIPROT #

Q03JI6

PROTEIN NAME

CRISPR-Cas9

SYNONYMS

Cas9 antibody;CRISPR-associated endonuclease Cas9/Csn1 antibody;CRISPR-Cas9/Csn1 antibody;csn1 antibody;SpyCas9 antibody

FUNCTION

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.

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