CRTC3 Recombinant Rabbit Monoclonal Antibody [JE63-18] (HA721887)
Catalog# HA721887
Rabbit Monoclonal to CRTC3
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CRTC3 on different lysates with Rabbit anti-CRTC3 antibody (HA721887) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: TT cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HDLM-2 cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 75 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721887) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: TT cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HDLM-2 cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 75 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721887) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.