Cyclin D3 Recombinant Rabbit Monoclonal Antibody [SD20-43] (ET1612-4)
Catalog# ET1612-4
Rabbit Monoclonal to Cyclin D3
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WB
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate
Lane 4: A20 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cyclin D3 Recombinant Rabbit Monoclonal Antibody [SD20-43] (ET1612-4)
Immunogen
Recombinant protein within human cyclin d3 aa 162-292 / 292.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, A20 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD20-43
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cyclin D3
SYNONYMS
CCND 3 antibody;Ccnd3 antibody;CCND3_HUMAN antibody;CyclinD3 antibody;D3 type cyclin antibody;G1 S specific cyclin D3 antibody;G1/S specific cyclin D3 antibody;G1/S-specific cyclin-D3 antibody
SEQUENCE SIMILARITIES
Belongs to the cyclin family. Cyclin D subfamily.
POST-TRANSLATIONAL MODIFICATION
Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm, Membrane.
FUNCTION
The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
CITATIONS
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Niu, M., Xu, J., Liu, Y., Li, Y., He, T., Ding, L., He, Y., Yi, Y., Li, F., Guo, R., Gao, Y., Li, R., Li, L., Fu, M., Hu, Q., Luo, Y., Zhang, C., Qin, K., Yi, J., Yu, S., … Xiao, Z. J.
FBXL2 counteracts Grp94 to destabilize EGFR and inhibit EGFR-driven NSCLC growth
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IHC
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Mouse
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Citation
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EZH2 expression and its role in spermatogonial stem cell self-renewal in goats
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WB
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Mouse
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Citation
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Knockdown of KDM5B Leads to DNA Damage and Cell Cycle Arrest in Granulosa Cells via MTF1
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WB
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Goat
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Citation
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