CYP27A1 Recombinant Rabbit Monoclonal Antibody [JG40-36] (ET7109-05)
Catalog# ET7109-05
Rabbit Monoclonal to CYP27A1
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: Mouse liver tissue lysate (20 µg/Lane)
Lane 3: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 50 kDa
Exposure time: Lane 1: 1 minute 2 seconds; Lane 2-3: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-05) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CYP27A1 Recombinant Rabbit Monoclonal Antibody [JG40-36] (ET7109-05)
Immunogen
Recombinant protein within human cyp27a1 aa 400-531 / 531.
Host
Rabbit
Positive Control
HepG2 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HepG2, human liver cancer tissue, mouse liver tissue, rat liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG40-36
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:100-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 60 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CYP27A1
SYNONYMS
12-alpha-triol 27-hydroxylase antibody;5-beta-cholestane-3-alpha antibody;5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody;5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody;5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody;7-alpha antibody;Cholestanetriol 26 monooxygenase antibody;CP27 antibody;CP27A_HUMAN antibody;CTX antibody;CYP antibody;CYP27 antibody;CYP27A1 antibody;Cytochrome P 450C27/25 antibody;Cytochrome P-450C27/25 antibody;Cytochrome P450 27 antibody;Cytochrome P450 27 mitochondrial antibody;Cytochrome P450 family 27 subfamily A member 1 antibody;Cytochrome P450 family 27 subfamily A polypeptide 1 antibody;Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody;mitochondrial antibody;Sterol 26 hydroxylase antibody;Sterol 26 hydroxylase mitochondrial antibody;Sterol 26-hydroxylase antibody;Sterol 27 hydroxylase antibody;Sterol 27-hydroxylase antibody;Vitamin D(3) 25 hydroxylase antibody;Vitamin D(3) 25-hydroxylase antibody
SEQUENCE SIMILARITIES
Belongs to the cytochrome P450 family.
TISSUE SPECIFICITY
Expressed in the neural retina and underlying retinal pigment epithelium (at protein level). Expressed in the gray and white matter of cerebellum (at protein level).
SUBCELLULAR LOCATION
Mitochondrion membrane.
FUNCTION
P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endo-genous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased aromatase activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7a-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver.
CITATIONS
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Dietary choline prevents high fat-induced disorder of hepatic cholesterol metabolism through SREBP-2/HNF-4α/CYP7A1 pathway in a freshwater teleost yellow catfish Pelteobagrus fulvidraco
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WB
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yellow catfish
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Citation
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Antagonizing EZH2 combined with vitamin D3 exerts a synergistic role in anti-fibrosis through bidirectional effects on hepatocytes and hepatic stellate cells
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WB
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Rat
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Citation
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