Skip to content

Contact

(857) 353-6600

orders@huabio.com

Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC69-08] (ET1610-60)

Price: Regular price $385.00

Volume

Bulk Order Quote   |   Customization Request

Catalog# ET1610-60

Rabbit Monoclonal to Cytochrome C

Tested for

  • WB

  • IF-Tissue

  • IHC-P

  • IP

  • IF-Cell

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

Select your chosen country/region

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC69-08] (ET1610-60)

Immunogen

Synthetic peptide within human cytochrome c aa 20-60.

Host

Rabbit

Positive Control

HeLa cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, Jurkat cell lysate, Human kidney tissue lysate, C6, human kidney tissue, mouse liver tissue, mouse kidney tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC69-08

RRID

AB_2940742

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IF-Tissue

  • 1:50-1:100

  • IHC-P

  • 1:1,000-1:5,000

  • IP

  • Use at an assay dependent concentration.

  • IF-Cell

  • 1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 12 kDa

Isotype

IgG

TARGET

UNIPROT #

P99999

PROTEIN NAME

Cytochrome C

SYNONYMS

CYC antibody;CYC_HUMAN antibody;CYCS antibody;Cytochrome c antibody;Cytochrome c somatic antibody;HCS antibody;THC4 antibody

SEQUENCE SIMILARITIES

Belongs to the cytochrome c family.

POST-TRANSLATIONAL MODIFICATION

Binds 1 heme group per subunit.; Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

SUBCELLULAR LOCATION

Mitochondrion intermembrane space.

FUNCTION

Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

CITATIONS

  • The HSP90 inhibitor KW-2478 depletes the malignancy of BCR/ABL and overcomes the imatinib-resistance caused by BCR/ABL amplification

  • Wang Ruixuan,et al

    Costunolide ameliorates MNNG-induced chronic atrophic gastritis through inhibiting oxidative stress and DNA damage via activation of Nrf2

  • Yang Fan,et al

    Engaging natural regulatory myeloid cells to restrict T-cell hyperactivation-induced liver inflammation via extracellular vesicle-mediated purine metabolism regulation

  • Jian Li; Caiqiao Zhang

    Metformin Prevents Follicular Atresia in Aging Laying Chickens through Activation of PI3K/AKT and Calcium Signaling Pathways

  • Bi Yanghui,et al

    Tumor-derived extracellular vesicle drug delivery system for chemo-photothermal-immune combination cancer treatment

  • Ma Yunhao,et al

    New findings on post-mortem chicken quality changes: The ROS-influenced MAPK-JNK signaling pathway affects chicken quality by regulating muscle cell apoptosis

  • Nanxin Zhang,et al

    Effects and mechanisms of Polygonati Rhizoma polysaccharide on potassium oxonate and hypoxanthine-induced hyperuricemia in mice

  • Yawen Wang ,et al

    Gold (I) complexes bearing EGFR-inhibiting ligands as anti-HCC agents through dual targeting of EGFR and TrxR

  • Zhao Q, He W, Liu Z, et al

    LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX

  • Long, X., Chen, L., Yang, J., Dong, T., Cheng, Q., Wang, W., Zou, Y., Su, Y., Dai, W., Chen, B., & Zhou, X.

    Network-based pharmacology and in vitro validation reveal that galangin induces apoptosis in bladder cancer cells by promoting the p53 signaling pathway

  • Combination of Zearalenone and Deoxynivalenol Induces Apoptosis by Mitochondrial Pathway in Piglet Sertoli Cells: Role of Endoplasmic Reticulum Stress

  • Li, Xin;Wang, Chunchun;Zhu, Jiang;Lin, Qian;Yu, Minjie;Wen, Jiashu;Feng, Jie;Hu, Caihong

    Sodium Butyrate Ameliorates Oxidative Stress-Induced Intestinal Epithelium Barrier Injury and Mitochondrial Damage through AMPK-Mitophagy Pathway

  • A network pharmacology approach and experimental validation to investigate the anticancer mechanism and potential active targets of ethanol extract of Wei-Tong-Xin against colorectal cancer through induction of apoptosis via PI3K/AKT signaling pathway

  • A New Fusion Peptide Targeting Pancreatic Cancer and Inhibiting Tumor Growth

  • Meiqi Wan,et al

    Rhein induces apoptosis of AGS and MGC803 cells by regulating the Ras/PI3K/AKT and p38/MAPK signaling pathway

  • Liu Yali,et al

    Quercetin Promotes Apoptosis of Gastric Cancer Cells through the EGFR-ERK Signaling Pathway

  • Wang Jinyu,et al

    Schisandra chinensis lignans regulate and cooperate with endogenous cannabinoid systems to ameliorate intestinal barrier injury associated with depression

  • Liu, X., Cui, S., Dan, C., Li, W., Xie, H., Li, C., & Shi, L.

    Phellinus baumii Polyphenol: A Potential Therapeutic Candidate against Lung Cancer Cells

  • Tang Xiaoli,et al

    Maternal exposure to beta-Cypermethrin disrupts placental development by dysfunction of trophoblast cells from oxidative stress

  • Chen Y, Wu Y, Zhou Y, et al

    The delaying effect of Qingxin Zishen decoction on ovarian aging: Examining regulation of ovarian mitochondria apoptosis in aged rats

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

Search

Main menu

Your cart is currently empty.
Shop now