Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody [SZ80-07] (ET1603-8)
Catalog# ET1603-8
Rabbit Monoclonal to Cytokeratin 18
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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mIHC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Cytokeratin 18 on different lysates with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/5,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-8) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-8) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
