Cytokeratin 19 Mouse Monoclonal Antibody [A3D1] (EM1901-75)
Catalog# EM1901-75
Mouse Monoclonal to Cytokeratin 19
Tested for
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WB
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IHC-P
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FC
Reacts with
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Human
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [A3D1] (EM1901-75) at 1/1,000 dilution.
Lane 1/2: Wild-type MCF-7 whole cell lysate (20 µg).
Lane 3/4: Cytokeratin 19 fragment 1 knockdown MCF-7 whole cell lysate (20 µg).
Lane 5/6: Cytokeratin 19 fragment 2 knockdown MCF-7 whole cell lysate (20 µg).
EM1901-75 was shown to specifically react with Cytokeratin 19 in wild-type MCF-7 cells. Weakened bands were observed when Cytokeratin 19 knockdown samples were tested. Wild-type and Cytokeratin 19 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM1901-75, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Lane 1/2: Wild-type MCF-7 whole cell lysate (20 µg).
Lane 3/4: Cytokeratin 19 fragment 1 knockdown MCF-7 whole cell lysate (20 µg).
Lane 5/6: Cytokeratin 19 fragment 2 knockdown MCF-7 whole cell lysate (20 µg).
EM1901-75 was shown to specifically react with Cytokeratin 19 in wild-type MCF-7 cells. Weakened bands were observed when Cytokeratin 19 knockdown samples were tested. Wild-type and Cytokeratin 19 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM1901-75, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
