Cytokeratin 20 Rabbit Polyclonal Antibody (ER1901-76)

UNIPROT #

P35900

PROTEIN NAME

Cytokeratin 20

SYNONYMS

CD20 antibody;CK 20 antibody;CK-20 antibody;CK20 antibody;Cytokeratin-20 antibody;Cytokeratin20 antibody;K1C20_HUMAN antibody;K20 antibody;KA20 antibody;Keratin 20 antibody;keratin 20, type I antibody;keratin 21, rat, homolog of antibody;Keratin antibody;Keratin type I cytoskeletal 20 antibody;Keratin-20 antibody;Keratin20 antibody;KRT 20 antibody;KRT 21 antibody;KRT20 antibody;KRT21 antibody;MGC35423 antibody;OTTHUMP00000164518 antibody;Protein IT antibody;type I cytoskeletal 20 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

DEVELOPMENTAL STAGE

First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.

POST-TRANSLATIONAL MODIFICATION

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).; Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epi-thelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition, they aid in the characterization of malignant tumors. Cytokeratin 20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract, and Cytokeratin 20 is a useful marker of pancreatic and colorectal cancer. Cytokeratin 20 is also helpful in distinguishing different types of highly related carcinomas, such as renal oncocytomas from renal cell carcinomas.