DARPP32 Recombinant Rabbit Monoclonal Antibody [SU0329] (ET1608-23)
Catalog# ET1608-23
Rabbit Monoclonal to DARPP32
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WB
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IF-Cell
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IP
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FC
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IHC-P
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IHC-Fr
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Human
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Mouse
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Rat
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1608-23, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
DARPP32 Recombinant Rabbit Monoclonal Antibody [SU0329] (ET1608-23)
Immunogen
Recombinant protein within human darpp32 aa 1-94 / 204.
Host
Rabbit
Positive Control
Mouse striatum tissue, mouse forebrain tissue, human brain tissue, human colon tissue, mouse brian tissue, mouse epididymis tissue, rat brian tissue, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat hippocampus tissue lysate, SH-SY5Y, MCF-7, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU0329
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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FC
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1:50-1:100
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IHC-P
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1:500-1:1,000
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IHC-Fr
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
DARPP32
SYNONYMS
DARPP 32 antibody;DARPP-32 antibody;Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody;Dopamine and cAMP regulated neuronal phosphoprotein antibody;Dopamine and cAMP regulated phosphoprotein antibody;Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody;Dopamine and cAMP regulated phosphoprotein DARPP32 antibody;Dopamine- and cAMP-regulated neuronal phosphoprotein antibody;FLJ20940 antibody;IPPD antibody;Neuronal phosphoprotein DARPP 32 antibody;PPP1R1B antibody;PPR1B_HUMAN antibody;Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody;Protein phosphatase 1 regulatory inhibitor subunit 1B antibody;Protein phosphatase 1 regulatory subunit 1B antibody
SEQUENCE SIMILARITIES
Belongs to the protein phosphatase inhibitor 1 family.
POST-TRANSLATIONAL MODIFICATION
Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.; Phosphorylation of Thr-34 is required for activity.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Protein phosphatase 1 regulatory subunit 1B (PPP1R1B), also known as dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP-32), is a protein that in humans is encoded by the PPP1R1B gene. Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders. One well studied target for the actions of dopamine is DARPP32. In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. DARPP-32 is critical for dopamine dependent striatal synaptic plasticity, possibly by serving as a dopamine-dependent gating mechanism for calcium/CaMKII signaling.
CITATIONS
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The involvement of ADAR1 in chronic unpredictable stress-induced cognitive impairment by targeting DARPP-32 with miR-874-3p in BALB/c mice
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WB
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Mouse
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Citation
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