DDR2 Recombinant Rabbit Monoclonal Antibody [JE31-45] (HA721479)
Catalog# HA721479
Rabbit Monoclonal to DDR2
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (40 µg/Lane)
Lane 2: SH-SY5Y cell lysate (40 µg/Lane)
Lane 3: L929 cell lysate (40 µg/Lane)
Lane 4: NIH/3T3 cell lysate (40 µg/Lane)
Lane 5: U-2 OS cell lysate (15 µg/Lane)
Lane 6: A375 cell lysate (15 µg/Lane)
Lane 7: PC-12 cell lysate (10 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721479) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
DDR2 Recombinant Rabbit Monoclonal Antibody [JE31-45] (HA721479)
Immunogen
Synthetic peptide within human ddr2 aa 806-855 / 855.
Host
Rabbit
Positive Control
HeLa cell lysate, SH-SY5Y cell lysate, L929 cell lysate, NIH/3T3 cell lysate, U-2 OS cell lysate, A375 cell lysate, PC-12 cell lysate, rat skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE31-45
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 97 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
DDR2
SYNONYMS
CD167 antigen-like family member B antibody;CD167b antibody;CD167b antigen antibody;Cell migration inducing protein 20 antibody;DDR 2 antibody;DDR2 antibody;DDR2_HUMAN antibody;Discoidin domain containing receptor 2 antibody;Discoidin domain receptor 2 antibody;Discoidin domain receptor family member 2 antibody;discoidin domain receptor tyrosine kinase 2 antibody;Discoidin domain-containing receptor 2 antibody;discoidin domain-containing receptor tyrosine kinase 2 antibody;Hydroxyaryl protein kinase antibody;MIG20a antibody;Migration inducing gene 16 protein antibody;Neurotrophic tyrosine kinase antibody;Neurotrophic tyrosine kinase receptor related 3 antibody;NTRKR 3 antibody;NTRKR3 antibody;Receptor protein tyrosine kinase TKT antibody;Receptor protein-tyrosine kinase TKT antibody;Receptor related 3 antibody;receptor-related 3 antibody;TKT antibody;TYRO 10 antibody;TYRO10 antibody;Tyrosine kinase receptor related to neurotrophic TRK antibody;Tyrosine protein kinase TYRO 10 antibody;Tyrosine protein kinase TYRO10 antibody;Tyrosine-protein kinase TYRO10 antibody;Tyrosylprotein kinase antibody
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
Discoidin domain-containing receptor 2, also known as CD167b (cluster of differentiation 167b), is a protein that in humans is encoded by the DDR2 gene. Discoidin domain-containing receptor 2 is a receptor tyrosine kinase (RTK). RTKs play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. In the case of DDR2, the ligand is collagen which binds to its extracellular discoidin domain. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. DDR2 has been associated with a number of diseases including fibrosis and cancer.
CITATIONS
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Duan Changling,et al
DNA methylation characteristics associated with chemotherapy resistance in epithelial ovarian cancer
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IHC
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Human
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Citation
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