DKK1 Recombinant Rabbit Monoclonal Antibody [SC06-86] (ET1610-63)
Catalog# ET1610-63
Rabbit Monoclonal to DKK1
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
-
Human
-
Rat
Lysates/proteins at 15 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 35-40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-63) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
DKK1 Recombinant Rabbit Monoclonal Antibody [SC06-86] (ET1610-63)
Immunogen
Synthetic peptide within human dkk1 aa 225-266 / 266.
Host
Rabbit
Positive Control
Caco-2 cell lysates, Hela, HepG2, NCCIT, human uterus tissue, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC06-86
RRID
APPLICATION DILUTION
-
WB
-
1:2,000
-
IF-Cell
-
1:50-1:500
-
IF-Tissue
-
1:50-1:500
-
IHC-P
-
1:50-1:1,000
-
FC
-
1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 29 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
DKK1
SYNONYMS
Dickkopf 1 antibody;Dickkopf 1 homolog antibody;Dickkopf 1 like antibody;Dickkopf homolog 1 antibody;Dickkopf like protein 1 antibody;Dickkopf related protein 1 antibody;Dickkopf WNT signaling pathway inhibitor 1 antibody;Dickkopf-1 antibody;Dickkopf-related protein 1 antibody;DKK 1 antibody;Dkk-1 antibody;Dkk1 antibody;DKK1_HUMAN antibody;hDkk 1 antibody;hDkk-1 antibody;SK antibody
SEQUENCE SIMILARITIES
Belongs to the dickkopf family.
TISSUE SPECIFICITY
Placenta.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the seven membrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-catenin and subsequent regulation of developmentally significant Wnt |target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-1 (Dickkopf-1) acts upstream of b-catenin and dishevelled to inhibit Wnt signaling. Dkk-1 is a 266-amino acid (human), secreted protein that contains a 31-residue N-terminal signal peptide, 2 cysteine rich domains, and a putative carboxy terminal N-glycosylation site. Human Dkk-1 transcripts are abundantly present in fetal kidney, adult placenta and adult prostate. Putative cis regulatory elements upstream of the Dkk-1 start site include p53, Sp1, MyoD, STAT, Oct-1/2, C/EBP-a, C/EBP-b, GATA-1, GATA-2 and GATA-3.
CITATIONS
-
Antagonizing EZH2 combined with vitamin D3 exerts a synergistic role in anti-fibrosis through bidirectional effects on hepatocytes and hepatic stellate cells
-
IF-tissue
-
WB
-
Rat
-
Citation
-
-
Julius Michael Wolfgart,et al
Biomarkers for hypertrophic chondrocyte differentiation are associated with spatial cellular organisation and suggest endochondral ossification-like processes in osteoarthritic cartilage: An exploratory study
-
Rabbit
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.