DNA-PKcs/PRKDC Mouse Monoclonal Antibody [2-B3] (M1204-6)

UNIPROT #

P78527

PROTEIN NAME

DNA-PKcs/PRKDC

SYNONYMS

DNA dependent protein kinase catalytic subunit antibody;DNA PK catalytic subunit antibody;DNA-dependent protein kinase catalytic subunit antibody;DNA-PK catalytic subunit antibody;DNA-PKcs antibody;DNAPK antibody;DNAPK catalytic subunit antibody;DNPK 1 antibody;DNPK1 antibody;Hyper radiosensitivity of murine scid mutation, complementing 1 antibody;Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody;HYRC 1 antibody;HYRC antibody;HYRC1 antibody;IMD26 antibody;p350 antibody;p460 antibody;PKRDC antibody;PRKDC antibody;PRKDC_HUMAN antibody;Protein Kinase DNA Activated Catalytic Polypeptide antibody;XRCC 7 antibody;XRCC7 antibody

SEQUENCE SIMILARITIES

Belongs to the PI3/PI4-kinase family.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated at two clusters, the T2609 cluster and the S2056 cluster (By similarity). Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair. Autophosphorylation of the T2609 cluster is required for hematopoietic development and protein synthesis in erythrocytes precursors (By similarity).; S-nitrosylated by GAPDH.; Polyubiquitinated by RNF144A, leading to proteasomal degradation.

SUBCELLULAR LOCATION

Nucleus, nucleolus.

FUNCTION

DNA-dependent protein kinase, catalytic subunit, also known as DNA-PKcs, is an enzyme that in humans is encoded by the gene designated as PRKDC or XRCC7. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. The DNA-Pkcs protein is a serine/threonine protein kinase comprising a single polypeptide chain of 4,128 amino acids. DNA-PKcs is the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase called DNA-PK. The second component is the autoimmune antigen Ku. On its own, DNA-PKcs is inactive and relies on Ku to direct it to DNA ends and trigger its kinase activity. DNA-PKcs is required for the non-homologous end joining (NHEJ) pathway of DNA repair, which rejoins double-strand breaks. It is also required for V(D)J recombination, a process that utilizes NHEJ to promote immune system diversity. DNA-PKcs knockout mice have severe combined immunodeficiency due to their V(D)J recombination defect. Many proteins have been identified as substrates for the kinase activity of DNA-PK. Autophosphorylation of DNA-PKcs appears to play a key role in NHEJ and is thought to induce a conformational change that allows end processing enzymes to access the ends of the double-strand break.[9] DNA-PK also cooperates with ATR and ATM to phosphorylate proteins involved in the DNA damage checkpoint.