DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62] (HA721993)
Catalog# HA721993
Rabbit Monoclonal to DOCK2
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of DOCK2 on different lysates with Rabbit anti-DOCK2 antibody (HA721993) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: THP-1 cell lysate
Lane 3: Raji cell lysate
Lane 4: 293T cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 212 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721993) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: THP-1 cell lysate
Lane 3: Raji cell lysate
Lane 4: 293T cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 212 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721993) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.