Dynamin 2 Recombinant Rabbit Monoclonal Antibody [JM61-38] (ET1705-2)
Catalog# ET1705-2
Rabbit Monoclonal to Dynamin 2
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: SK-OV-3 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: PC-12 cell lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Lane 8: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 98 kDa
Observed band size: 98 kDa
Exposure time: 28 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-2) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Dynamin 2 Recombinant Rabbit Monoclonal Antibody [JM61-38] (ET1705-2)
Immunogen
Synthetic peptide within human dynamin 2 aa 748-797 / 870.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, PC-12, human colon tissue, mouse colon tissue, rat colon tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM61-38
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:200-1:1,000
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 98 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Dynamin 2
SYNONYMS
CMT2M antibody;CMTDI1 antibody;CMTDIB antibody;DI CMTB antibody;Dnm2 antibody;DYN II antibody;DYN2 antibody;DYN2_HUMAN antibody;Dynamin II antibody;Dynamin-2 antibody;Dynamin2 antibody;DynaminII antibody;DYNII antibody
SEQUENCE SIMILARITIES
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.
TISSUE SPECIFICITY
Ubiquitously expressed.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by calcineurin/PP2 (By similarity). Phosphorylated on tyrosine residues after activation of SRC (By similarity).
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Dynamin-2 is a protein that in humans is encoded by the DNM2 gene. Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.
CITATIONS
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Dynamics of transmissible gastroenteritis virus internalizationunraveled by single-virus tracking in live cells
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WB
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Pig
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Citation
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