E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287] (ET1607-75)
Catalog# ET1607-75
Rabbit Monoclonal to E-Cadherin
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
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mIHC
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Human
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (ET1607-75) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287] (ET1607-75)
Immunogen
Synthetic peptide within human e-cadherin aa 580-630 (extracellular).
Host
Rabbit
Positive Control
MCF7 cell lysate, HT-29 cell lysate, HCT 116 cell lysate, A431 cell lysate, Caco-2 cell lysate, HT-29, human lung carcinoma tissue, human colon tissue, human breast cancer tissue, human tonsils tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY0287
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:2,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
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mIHC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 97 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
E-Cadherin
SYNONYMS
Arc 1 antibody;CADH1_HUMAN antibody;Cadherin 1 antibody;cadherin 1 type 1 E-cadherin antibody;Cadherin1 antibody;CAM 120/80 antibody;CD 324 antibody;CD324 antibody;CD324 antigen antibody;cdh1 antibody;CDHE antibody;E-Cad/CTF3 antibody;E Cadherin antibody;E-cadherin antibody;ECAD antibody;Epithelial cadherin antibody;epithelial calcium dependant adhesion protein antibody;LCAM antibody;Liver cell adhesion molecule antibody;UVO antibody;Uvomorulin antibody
TISSUE SPECIFICITY
Non-neural epithelial tissues.
POST-TRANSLATIONAL MODIFICATION
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation (By similarity).; N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location.; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.; O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Thr-285 and Thr-509 are O-mannosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3.
SUBCELLULAR LOCATION
Cell junction, Cell membrane, Endosome, Golgi apparatus.
FUNCTION
Cadherin-1 (not to be confused with the APC/C activator protein CDH1) also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene. Mutations are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. CDH1 has also been designated as CD324 (cluster of differentiation 324). It is a tumor suppressor gene. E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. In epithelial cells, E-cadherin-containing cell-to-cell junctions are often adjacent to actin-containing filaments of the cytoskeleton. E-cadherin is first expressed in the 2-cell stage of mammalian development, and becomes phosphorylated by the 8-cell stage, where it causes compaction.
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