E2F1 Recombinant Rabbit Monoclonal Antibody [JJ092-02] (ET1701-73)
Catalog# ET1701-73
Rabbit Monoclonal to E2F1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
Lane 1: HT-29 cell lysate
Lane 2: SW480 cell lysate
Lane 3: SW620 cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-73) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
E2F1 Recombinant Rabbit Monoclonal Antibody [JJ092-02] (ET1701-73)
Immunogen
Synthetic peptide within human e2f1 aa 371-404 / 437.
Host
Rabbit
Positive Control
HT-29 cell lysate, SW480 cell lysate, SW620 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, rat heart tissue lysate, Hela, MCF-7, HepG2, human tonsil tissue, mouse pancreas tissue, rat heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ092-02
RRID
APPLICATION DILUTION
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WB
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1:500-1:10,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:1,000-1:5,000
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FC
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1:1,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 47 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
E2F1
SYNONYMS
DmelCG6376 antibody;Dmel_CG6376 antibody;drosE2F1 antibody;E(Sev-CycE)3A antibody;E(var)3-93E antibody;E2-promoter binding facto antibody;E2F 1 antibody;E2F transcription factor 1 antibody;E2F-1 antibody;E2f-PA antibody;E2f-PB antibody;E2f-PC antibody;E2F1 antibody;E2f1 E2F transcription factor 1 antibody;E2F1_HUMAN antibody;Evar(3)164 antibody;KIAA4009 antibody;l(3)07172 antibody;l(3)j3B1 antibody;l(3)j3C2 antibody;l(3)rM729 antibody;mKIAA4009 antibody;OTTHUMP00000030661 antibody;PBR3 antibody;PRB binding protein E2F 1 antibody;PRB-binding protein E2F-1 antibody;RBAP 1 antibody;RBAP-1 antibody;RBAP1 antibody;RBBP-3 antibody;RBBP3 antibody;RBP 3 antibody;RBP3 antibody;Retinoblastoma-associated protein 1 antibody;Retinoblastoma-binding protein 3 antibody;Transcription factor E2F1 antibody
SEQUENCE SIMILARITIES
Belongs to the E2F/DP family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis.; Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.
CITATIONS
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MicroRNA-31 regulates dental epithelial cell proliferation by targeting Satb2
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WB
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Mouse
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Citation
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Chinese Propolis Inhibits the Proliferation of Human Gastric Cancer Cells by Inducing Apoptosis and Cell Cycle Arrest
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WB
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Human
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Citation
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Liu, X., Cui, S., Dan, C., Li, W., Xie, H., Li, C., & Shi, L.
Phellinus baumii Polyphenol: A Potential Therapeutic Candidate against Lung Cancer Cells
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WB
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Human
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Citation
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