EEA1 Mouse Monoclonal Antibody [B0-A8] (M1503-1)
Catalog# M1503-1
Mouse Monoclonal to EEA1
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WB
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IHC-P
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Human
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Mouse
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Rat
Western blot analysis of EEA1 on different lysates with Mouse anti-EEA1 antibody (M1503-1) at 1/10,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-EEA1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 162 kDa
Observed band size: 162 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1503-1) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
EEA1 Mouse Monoclonal Antibody [B0-A8] (M1503-1)
Immunogen
Synthetic peptide of the n terminal residues of human eea1.
Host
Mouse
Positive Control
HeLa cell lysate, Daudi cell lysate, A431 cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, C6 cell lysate, human prostate tissue, mouse prostate tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
B0-A8
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:10,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 162 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
EEA1
SYNONYMS
Early endosome antigen 1 antibody;Early endosome antigen 1, 162kD antibody;Early endosome associated protein antibody;EEA 1 antibody;EEA1 antibody;EEA1_HUMAN antibody;Endosome associated protein p162 antibody;Endosome-associated protein p162 antibody;MST105 antibody;MSTP105 antibody;ZFYVE2 antibody;Zinc finger FYVE domain containing protein 2 antibody;Zinc finger FYVE domain-containing protein 2 antibody
SUBCELLULAR LOCATION
Cytoplasm, early endosome membrane.
FUNCTION
EEA1 localizes exclusively to early endosomes?and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate?through its C-terminalFYVE domain?and forms a homodimer?through a?coiled coil. EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as?N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. Due to the proteins importance in vesicular trafficking, a number of intracellular bacteria prevent EEA1 recruitment to the vacuole.Mycobacterium tuberculosis?is known to inhibit the recruitment of EEA1 to the phagosomal membrane through CamKII. Legionella pneumophila?also prevents EEA1 recruitment through a currently unknown mechanism.[5] Interestingly, the related pathogen Legionella longbeachae?recruits EEA1 and appears to replicate within a modified early endosome.
CITATIONS
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Liu, Q., Luo, Y., Zhao, Y., Xiang, P., Zhu, J., Jing, W., Jin, W., Chen, M., Tang, R., & Yu, H.
Nano-hydroxyapatite accelerates vascular calcification via lysosome impairment and autophagy dysfunction in smooth muscle cells
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WB
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Human
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Citation
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