eIF3A Recombinant Rabbit Monoclonal Antibody [JE58-60] (HA721375)
Catalog# HA721375
Rabbit Monoclonal to eIF3A
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of eIF3A on different lysates with Rabbit anti-eIF3A antibody (HA721375) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: A431 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Raji cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: L6 cell lysate
Lane 8: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 167 kDa
Observed band size: 167 kDa
Exposure time: 7seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721375) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: A431 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Raji cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: L6 cell lysate
Lane 8: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 167 kDa
Observed band size: 167 kDa
Exposure time: 7seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721375) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.