ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44] (HA721194)
Catalog# HA721194
Rabbit Monoclonal to ErbB3/HER3
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IHC-P
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IF-Tissue
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Human
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721194) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44] (HA721194)
Immunogen
Synthetic peptide within human erbb3 aa 650-750 (internal sequence).
Host
Rabbit
Positive Control
Human small intestine tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PD00-44
RRID
APPLICATION DILUTION
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IHC-P
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1:2,000
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 148 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ErbB3/HER3
SYNONYMS
c erbB 3 antibody;c erbB3 antibody;Erb b2 receptor tyrosine kinase 3 antibody;ErbB 3 antibody;ERBB3 antibody;ERBB3 protein antibody;erbB3 S antibody;ERBB3_HUMAN antibody;Glial growth factor receptor antibody;HER 3 antibody;HER3 antibody;Human epidermal growth factor receptor 3 antibody;LCCS2 antibody;MDA BF 1 antibody;MGC88033 antibody;p180 ErbB3 antibody;p45 sErbB3 antibody;p85 sErbB3 antibody;proto-oncogene-like protein c ErbB 3 antibody;proto-oncogene-like protein c ErbB3 antibody;Proto-oncogene-like protein c-ErbB-3 antibody;Receptor tyrosine protein kinase erbB 3 antibody;Receptor tyrosine protein kinase erbB3 antibody;Receptor tyrosine-protein kinase erbB-3 antibody;Tyrosine kinase type cell surface receptor HER3 antibody;Tyrosine kinase-type cell surface receptor HER3 antibody;v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody;v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody;v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
SUBCELLULAR LOCATION
Cell membrane, Secreted.
FUNCTION
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
CITATIONS
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Identification of a Hypoxia-Related Signature for Predicting Prognosis and the Immune Microenvironment in Bladder Cancer
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IHC
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Citation
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