ERK1 Recombinant Rabbit Monoclonal Antibody [SP00-74] (ET1604-32)
Catalog# ET1604-32
Rabbit Monoclonal to ERK1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A549 cell lysate
Lane 4: Ramos cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-32) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ERK1 Recombinant Rabbit Monoclonal Antibody [SP00-74] (ET1604-32)
Immunogen
Synthetic peptide within n-terminal human erk1.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, Ramos cell lysate, MCF7 cell lysate, Jurkat, human breast cancer tissue, human stomach tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SP00-74
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100
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IF-Tissue
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1:100-1:500
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IHC-P
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1:1,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ERK1
SYNONYMS
ERK 1 antibody;ERK antibody;ERK-1 antibody;ERK1 antibody;ERT 2 antibody;ERT2 antibody;Extracellular Signal Regulated Kinase 1 antibody;Extracellular signal related kinase 1 antibody;Extracellular signal-regulated kinase 1 antibody;HGNC6877 antibody;HS44KDAP antibody;HUMKER1A antibody;Insulin Stimulated MAP2 Kinase antibody;Insulin-stimulated MAP2 kinase antibody;MAP kinase 1 antibody;MAP kinase 3 antibody;MAP Kinase antibody;MAP kinase isoform p44 antibody;MAPK 1 antibody;MAPK 3 antibody;MAPK antibody;MAPK1 antibody;Mapk3 antibody;MGC20180 antibody;Microtubule Associated Protein 2 Kinase antibody;Microtubule-associated protein 2 kinase antibody;Mitogen Activated Protein Kinase 3 antibody;Mitogen-activated protein kinase 1 antibody;Mitogen-activated protein kinase 3 antibody;MK03_HUMAN antibody;OTTHUMP00000174538 antibody;OTTHUMP00000174541 antibody;p44 ERK1 antibody;p44 MAPK antibody;p44-ERK1 antibody;p44-MAPK antibody;P44ERK1 antibody;P44MAPK antibody;PRKM 3 antibody;PRKM3 antibody;Protein Kinase Mitogen Activated 3 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p12-p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2.
CITATIONS
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Wen, J., Bao, Z., Li, L., Liu, Y., Wei, B., Ye, X., Xu, H., Cui, L., Li, X., Shen, G., Fang, Y., Zeng, H., Shen, Z., Guo, E., Jin, H., & Wu, L.
Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects
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IHC
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Mouse
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Citation
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Zhou, S., Ma, Y., Yao, J., Zhao, A., Xie, C., Mi, Y., & Zhang, C.
TGF-β1-induced collagen promotes chicken ovarian follicle development via an intercellular cooperative pattern
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WB
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Hen
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Citation
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Tianfu Wen,Chuan Li;
Loss of FoxA2 accelerates neoplastic changes in the intrahepatic bile duct partly via the MAPK signaling pathway
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WB
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IHC
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human
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Citation
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