ERp29 Recombinant Rabbit Monoclonal Antibody [JG34-42] (ET7108-03)
Catalog# ET7108-03
Rabbit Monoclonal to ERp29
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WB
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IHC-P
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
Western blot analysis of ERp29 on different lysates with Rabbit anti-ERp29 antibody (ET7108-03) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate (10 µg/Lane)
Lane 2: HAP1-ERp29 KD cell lysate (10 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-03) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ERp29 Recombinant Rabbit Monoclonal Antibody [JG34-42] (ET7108-03)
Immunogen
Recombinant protein within human erp29 aa 1-142 / 261.
Host
Rabbit
Positive Control
THP-1 cell lysate, F9, PMVEC, SiHa, rat testis tissue, human thyroid gland tissue, human colon cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG34-42
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 29 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ERp29
SYNONYMS
C12orf8 antibody;Chromosome 12 open reading frame 8 antibody;Endoplasmic reticulum lumenal protein ERp28 antibody;Endoplasmic reticulum protein 29 antibody;Endoplasmic reticulum protein 29 isoform 1 antibody;Endoplasmic reticulum protein ERp29 antibody;Endoplasmic reticulum resident protein 28 antibody;Endoplasmic reticulum resident protein 29 antibody;Endoplasmic reticulum resident protein 31 antibody;Epididymis secretory protein Li 107 antibody;ERp28 antibody;ERP29_HUMAN antibody;ERp31 antibody;HEL S 107 antibody;PDI-DB antibody;PDIA9 antibody;Protein disulfide isomerase family A member 9 antibody
TISSUE SPECIFICITY
Ubiquitous. Mostly expressed in secretory tissues.
SUBCELLULAR LOCATION
Endoplasmic reticulum.
FUNCTION
ERp29 is a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide-isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.
CITATIONS
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Cao, X., Shu, Y., Chen, Y., Xu, Q., Guo, G., Wu, Z., Shao, M., Zhou, Y., Chen, M., Gong, Y., Li, C., Shi, Y., & Bu, H.
Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.
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WB
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Mouse
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Citation
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