Ezrin Recombinant Rabbit Monoclonal Antibody [JM10-65] (ET1703-25)

UNIPROT #

P15311

PROTEIN NAME

Ezrin

SYNONYMS

Villin 2 ezrin antibody;CVIL antibody;CVL antibody;Cytovillin 2 antibody;Cytovillin antibody;DKFZp762H157 antibody;Epididymis secretory protein Li 105 antibody;EZR antibody;EZRI_HUMAN antibody;Ezrin antibody;FLJ26216 antibody;HEL S 105 antibody;MGC1584 antibody;p81 antibody;VIL 2 antibody;VIL2 antibody;Villin 2 (ezrin) antibody;Villin 2 antibody;Villin-2 antibody;Villin2 antibody

TISSUE SPECIFICITY

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

DEVELOPMENTAL STAGE

Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

SUBCELLULAR LOCATION

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.

FUNCTION

Ezrin also known as cytovillin or villin-2 is a protein that in humans is encoded by the EZR gene. The cytoplasmic peripheral protein encoded by this gene can be phosphorylated by protein-tyrosine kinase in microvilli and is a member of the ERM protein family. This protein serves as a linker between plasma membrane and actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. The N-terminal domain (also called FERM domain) binds sodium-hydrogen exchanger regulatory factor (NHERF) protein (involving long-range allostery). This binding can happen only when ezrin is in its active state. The activation of ezrin occurs in synergism of the two factors: 1) binding of the N-terminal domain to phosphatidylinositol(4,5)bis-phosphate (PIP2) and 2) phosphorylation of threonine T567 in the C-terminal domain. Binding to actin filaments (via C-terminal) and to membrane proteins (via N-terminal) stabilizes the protein's conformation in its active mode. The membrane proteins like CD44 and ICAM-2 are indirect binding partners of ezrin, while EBP50 (ERM binding protein 50) can associate with ezrin directly.