Fatty Acid Synthase Rabbit Polyclonal Antibody (R1706-8)
Catalog# R1706-8
Rabbit Polyclonal to Fatty Acid Synthase
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
All lanes: Western blot analysis of Fatty Acid Synthase with anti-Fatty Acid Synthase antibody (R1706-8) at 1:1,000 dilution.
Lane 1: Wild-type Hela whole cell lysate.
Lane 2: FASN knockdown Hela whole cell lysate.
R1706-8 was shown to specifically react with FASN in wild-type Hela cells. Weakened band was observed when FASN knockdown samples were tested. Wild-type and FASN knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-FASN antibody (R1706-8, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Fatty Acid Synthase Rabbit Polyclonal Antibody (R1706-8)
Immunogen
Recombinant protein within human fatty acid synthase aa 801-1050.
Host
Rabbit
Positive Control
A549, MCF-7, Hela, human prostate tissue, human breast cancer tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
273 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Fatty Acid Synthase
SYNONYMS
[Acyl-carrier-protein] S acetyltransferase antibody;[Acyl-carrier-protein] S malonyltransferase antibody;3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody;3-oxoacyl-[acyl-carrier-protein] reductase antibody;3-oxoacyl-[acyl-carrier-protein] synthase antibody;Enoyl-[acyl-carrier-protein] reductase antibody;FAS antibody;FAS_HUMAN antibody;FASN antibody;Fatty acid synthase antibody;MGC14367 antibody;MGC15706 antibody;OA 519 antibody;Oleoyl-[acyl-carrier-protein] hydrolase antibody;SDR27X1 antibody;Short chain dehydrogenase/reductase family 27X member 1 antibody
TISSUE SPECIFICITY
Ubiquitous. Prominent expression in brain, lung, and liver.
SUBCELLULAR LOCATION
Cytoplasm. Melanosome.
FUNCTION
Fatty acid biosynthesis is mediated by seven catalytic enzymes and an acyl carrier protein (ACP), to which various acyl intermediates are covalently attached. Fatty Acid Synthase (FAS) is the anabolic enzyme that contains the seven unique catalytic sites and mediates the conversion of acetyl-CoA and malonyl-CoA, in the presence of the cofactor NADPH, into long-chain saturated fatty acids, such as palmitate. Human Fatty Acid Synthase cDNA encodes a 2,504 amino acid protein. Catalytically active Fatty Acid Synthase is a homodimer. Human Fatty Acid Synthase mRNA is variably expressed with abundant levels present in brain, lung and liver. Fatty acid synthetic metabolism is abnormally elevated in tumor cells and may support cell growth or survival of malignant cancers.
CITATIONS
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Shen, J., Huang, J., Huang, Y., Chen, Y., Li, J., Luo, P., Zhang, Q., Qiu, Y., Wang, L., Jiang, H., Ma, S., & Chen, X.
Anlotinib suppresses lung adenocarcinoma growth via inhibiting FASN-mediated lipid metabolism
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WB
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IHC-P
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Mouse
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Citation
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Li, H., Liu, N. N., Li, J. R., Dong, B., Wang, M. X., Tan, J. L., Wang, X. K., Jiang, J., Lei, L., Li, H. Y., Sun, H., Jiang, J. D., & Peng, Z. G.
Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease
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WB
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Mouse
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Citation
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