Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36] (ET1705-55)
Catalog# ET1705-55
Rabbit Monoclonal to Ferritin Heavy Chain
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IHC-P
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Human
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36] (ET1705-55)
Immunogen
Synthetic peptide within c-terminal human ferritin heavy chain.
Host
Rabbit
Positive Control
Human kidney tissue, human liver tissue, human placenta tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM22-36
RRID
APPLICATION DILUTION
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IHC-P
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1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 21 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Ferritin Heavy Chain
SYNONYMS
Apoferritin antibody;Cell proliferation inducing gene 15 protein antibody;Cell proliferation-inducing gene 15 protein antibody;F HC antibody;Ferritin H subunit antibody;Ferritin heavy chain antibody;Ferritin heavy polypeptide 1 antibody;FHC antibody;FRIH antibody;FRIH_HUMAN antibody;FTH 1 antibody;FTH antibody;FTH1 antibody;FTH1 protein antibody;FTHL 6 antibody;FTHL6 antibody;Iron overload autosomal dominant antibody;MGC104426 antibody;N-terminally processed antibody;OK/SW-cl.84 antibody;PIG 15 antibody;PIG15 antibody;Placenta immunoregulatory factor antibody;PLIF antibody;Proliferation inducing gene 15 protein antibody;Proliferation inducing protein 15 antibody
SEQUENCE SIMILARITIES
Belongs to the ferritin family.
TISSUE SPECIFICITY
Expressed in the liver.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
Mammalian ferritins consist of 24 subunits made up of two types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of FeII, whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of FeIII. The most prominent role of mamma-lian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.
CITATIONS
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Chen Ying,et al
Cpd-A1 alleviates acute kidney injury by inhibiting ferroptosis
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WB
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Mouse
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Citation
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Sun, Y., He, Y., Tong, J., Liu, D., Zhang, H., He, T., & Bi, Y.
All-trans retinoic acid inhibits the malignant behaviors of hepatocarcinoma cells by regulating ferroptosis
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WB
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Human
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Citation
-
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Zhao Jingyuan,et al
Heme oxygenase-1 is involved in the erastin-induced ferroptosis in acute myeloid leukemia cells
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WB
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Human
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Citation
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