FIP200 Mouse Monoclonal Antibody [A9A10] (HA601107)
Catalog# HA601107
Mouse Monoclonal to FIP200
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Western blot analysis of FIP200 on different lysates with Mouse anti-FIP200 antibody (HA601107) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: MG-63 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: 293T cell lysate
Lane 5: K562 cell lysate
Lane 6: A431 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: HepG2 cell lysate
Lane 9: NIH/3T3 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 183 kDa
Observed band size: 250/150 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601107) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate
Lane 2: MG-63 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: 293T cell lysate
Lane 5: K562 cell lysate
Lane 6: A431 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: HepG2 cell lysate
Lane 9: NIH/3T3 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 183 kDa
Observed band size: 250/150 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601107) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.