FOXL2 Recombinant Rabbit Monoclonal Antibody [JE38-52] (HA721558)
Catalog# HA721558
Rabbit Monoclonal to FOXL2
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WB
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IHC-P
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Human
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Mouse
Lane 1: THP-1 cell lysate
Lane 2: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721558) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
FOXL2 Recombinant Rabbit Monoclonal Antibody [JE38-52] (HA721558)
Immunogen
Synthetic peptide within human foxl2 aa 1-50 / 376.
Host
Rabbit
Positive Control
THP-1 cell lysate, K-562 cell lysate, human ovary tissue, mouse ovary tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE38-52
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 39 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
FOXL2
SYNONYMS
Blepharophimosis antibody;Blepharophimosis epicanthus inversus and ptosis 1 antibody;Blepharophimosis epicanthus inversus and ptosis antibody;BPES 1 antibody;BPES antibody;BPES1 antibody;Epicanthus inversus and ptosis 1 antibody;Forkhead box L2 antibody;Forkhead box protein L2 antibody;Forkhead transcription factor FOXL2 antibody;FOX L2 antibody;FOXL 2 antibody;FOXL2 antibody;FOXL2_HUMAN antibody;PFRK antibody;PINTO antibody;PITUITARY FORKHEAD FACTOR antibody;POF 3 antibody;POF3 antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
CITATIONS
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Foxl2 is required for the initiation of the female pathway in a temperature-dependent sex determination system in Trachemys scripta
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IF
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Turtles
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Citation
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