FRA1 Recombinant Rabbit Monoclonal Antibody [JE33-20] (HA721447)
Catalog# HA721447
Rabbit Monoclonal to FRA1
Tested for
-
WB
-
IHC-P
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IF-Cell
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FC
-
ChIP
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of FRA1 on different lysates with Rabbit anti-FRA1 antibody (HA721447) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (negative) (20 µg/Lane)
Lane 5: HEK-293 cell lysate (negative) (20 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 37 kDa (40 kDa unknown)
Exposure time: 35 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721447) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FRA1 on different lysates with Rabbit anti-FRA1 antibody (HA721447) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (negative) (20 µg/Lane)
Lane 5: HEK-293 cell lysate (negative) (20 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 37 kDa (40 kDa unknown)
Exposure time: 35 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721447) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.