GAD67 Recombinant Rabbit Monoclonal Antibody [JM11-11] (ET1703-71)
Catalog# ET1703-71
Rabbit Monoclonal to GAD67
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WB
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IP
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GAD67 Recombinant Rabbit Monoclonal Antibody [JM11-11] (ET1703-71)
Immunogen
Synthetic peptide within human gad67 aa 60-99 / 594.
Host
Rabbit
Positive Control
HeLa cell lysate, SH-SY5Y cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-11
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IP
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1:50-1:100
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 67 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GAD67
SYNONYMS
67 kDa glutamic acid decarboxylase antibody;CPSQ1 antibody;DCE1 antibody;DCE1_HUMAN antibody;EC 4.1.1.15 antibody;FLJ45882 antibody;GAD 67 antibody;GAD antibody;GAD-67 antibody;GAD1 antibody;Glutamate decarboxylase 1 (brain, 67kDa) antibody;Glutamate decarboxylase 1 antibody;Glutamate decarboxylase 1 brain 67kD antibody;Glutamate decarboxylase 1 brain 67kDa antibody;Glutamate decarboxylase 67 kDa isoform antibody;Glutamate decarboxylase, brain, 67-KD antibody;OTTHUMP00000041055 antibody;SCP antibody
SEQUENCE SIMILARITIES
Belongs to the group II decarboxylase family.
TISSUE SPECIFICITY
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
SUBCELLULAR LOCATION
Cytoplasm, Plasma Membrane.
FUNCTION
There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (g-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67, the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues.
CITATIONS
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Jian Wang,et al
The modulation of cholecystokinin receptor 1 in the NAc core input from VTA on METH-induced CPP acquisition
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IF
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Mouse
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Citation
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