Gephyrin Recombinant Rabbit Monoclonal Antibody [JG34-47] (ET7108-04)
Catalog# ET7108-04
Rabbit Monoclonal to Gephyrin
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Gephyrin on different lysates with Rabbit anti-Gephyrin antibody (ET7108-04) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate (15 µg/Lane)
Lane 2: Neuro-2a cell lysate (15 µg/Lane)
Lane 3: C6 cell lysate (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (30 µg/Lane)
Lane 5: Mouse liver tissue lysate (30 µg/Lane)
Lane 6: Mouse kidney tissue lysate (30 µg/Lane)
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-Br-3 cell lysate (15 µg/Lane)
Lane 2: Neuro-2a cell lysate (15 µg/Lane)
Lane 3: C6 cell lysate (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (30 µg/Lane)
Lane 5: Mouse liver tissue lysate (30 µg/Lane)
Lane 6: Mouse kidney tissue lysate (30 µg/Lane)
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.