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GFP Mouse Monoclonal Antibody [2-D8] (EM30501)

Price: Regular price $350.00

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Catalog# EM30501

Mouse Monoclonal to GFP

Tested for

  • WB

  • IF-Cell

  • ELISA

  • FC

  • IP

Reacts with

  • Species independent

This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Mouse monoclonal primary

Product Name

GFP Mouse Monoclonal Antibody [2-D8] (EM30501)

Immunogen

Recombinant full length protein of aequorea victoria gfp.

Host

Mouse

Positive Control

Transfected K562 with GFP.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2-D8

RRID

AB_3068992

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IF-Cell

  • 1:200

  • IP

  • 2-5 µg/ml.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 27 kDa

Isotype

IgG1

TARGET

UNIPROT #

P42212

PROTEIN NAME

GFP

SYNONYMS

GFP antibody;Green fluorescent protein antibody;yfp antibody

SEQUENCE SIMILARITIES

Belongs to the GFP family.

TISSUE SPECIFICITY

Photocytes.

POST-TRANSLATIONAL MODIFICATION

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

FUNCTION

Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.

CITATIONS

  • Tang Y

    Successful construction and stable expression of an anti-CD45RA scFv–EGFP fusion protein in Chinese hamster ovary cells

  • HFE inhibits type I IFNs signaling by targeting the SQSTM1-mediated MAVS autophagic degradation

  • Liang Xue,et al

    Suppressing FXR promotes antiviral effects of bile acids via enhancing the interferon transcription

  • Tao Zhihuan,et al

    HD-ZIP IV Gene ROC1 Regulates Leaf Rolling and Drought Response Through Formation of Heterodimers with ROC5 and ROC8 in Rice

  • Fengming Song

    Overexpression of MoSM1,encoding for an immunity-inducing protein from Magnaporthe oryzae,in rice confers broad-spectrum resistance against fungal and bacterial diseases

  • The E3 ubiquitin ligase OsRGLG5 targeted by the Magnaporthe oryzae effector AvrPi9 confers basal resistance against rice blast

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