Glutamine Synthetase Mouse Monoclonal Antibody [A3G2] (EM1902-39)
Catalog# EM1902-39
Mouse Monoclonal to Glutamine Synthetase
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WB
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IHC-P
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FC
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mIHC
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: SK-Br-3 cell lysate
Lane 8: Human liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-39) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Glutamine Synthetase Mouse Monoclonal Antibody [A3G2] (EM1902-39)
Immunogen
Recombinant protein within human glutamine synthetase aa 190-373.
Host
Mouse
Positive Control
HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, SK-Br-3 cell lysate, human liver tissue lysate, human spleen tissue, mouse liver tissue, rat liver tissue, THP-1.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3G2
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:500-1:2,000
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FC
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1:50-1:100
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mIHC
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1:2,000
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IF-Tissue
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1:200-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG2a
TARGET
UNIPROT #
PROTEIN NAME
Glutamine Synthetase
SYNONYMS
cell proliferation-inducing protein 59 antibody;Cgl2214 antibody;GLNA antibody;GLNA_HUMAN antibody;GLNS antibody;GLUL antibody;Glutamate ammonia ligase antibody;Glutamate decarboxylase antibody;Glutamate--ammonia ligase antibody;glutamine synthase antibody;Glutamine synthetase antibody;glutamine synthetase I antibody;GS antibody;PIG 43 antibody;PIG 59 antibody;PIG43 antibody;PIG59 antibody;Proliferation inducing protein 43 antibody
SUBCELLULAR LOCATION
Microsome, Cytosol, Mitochondrion, Cell membrane.
FUNCTION
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.
CITATIONS
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The Cytidine N-Acetyltransferase NAT10 Participates in Peripheral Nerve Injury-Induced Neuropathic Pain by Stabilizing SYT9 Expression in Primary Sensory Neurons
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IF
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Mouse
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Citation
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