GPR56 Recombinant Rabbit Monoclonal Antibody [PSH05-43] (HA722273)
Catalog# HA722273
Rabbit Monoclonal to GPR56
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of GPR56 on different lysates with Rabbit anti-GPR56 antibody (HA722273) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: A-172 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: T-47D cell lysate
Lane 4: Human liver tissue lysate (negative)
Lane 5: Human brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 55-70 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722273) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A-172 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: T-47D cell lysate
Lane 4: Human liver tissue lysate (negative)
Lane 5: Human brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 55-70 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722273) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.