GST, AlpHcAbs® Rabbit antibody Alpaca Monoclonal (HA710107)
Catalog# HA710107
Alpaca Monoclonal to GST
Tested for
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WB
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ELISA
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IP
Western blot analysis of GST tag on different lysates with Rabbit anti-GST tag antibody (HA710107) at 1/5,000 dilution.
Lane 1: 293T transfected with GST cell lysate
Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710107) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with GST cell lysate
Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710107) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.