HRP Conjugated GST Rabbit Polyclonal Antibody (1006-3)
Catalog# 1006-3
Rabbit Polyclonal to GST
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WB
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ELISA
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Species independent
Lane 1: 293T cell lysate
Lane 2: 293T transfected with GST cell lysate
Lane 3: 293T transfected with GST-tagged Histone H3.1 cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1006-3) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
HRP Conjugated GST Rabbit Polyclonal Antibody (1006-3)
Immunogen
Recombinant protein within gst protein.
Host
Rabbit
Conjugation
Hrp
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:10,000
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ELISA
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1:10,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
GST
SYNONYMS
Glutathione S Transferase antibody;Glutathione S-transferase class-mu 26 kDa isozyme antibody;GST antibody;Sj26 antigen antibody;SjGST antibody
FUNCTION
The pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots, as well as for immunogen affinity purification of proteins expressed in E. coli.
CITATIONS
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Yue, Z. L., Tian, Z. J., Zhang, J. W., Zhang, S. W., Li, Y. D., & Wu, Z. M.
Overexpression of Lectin Receptor-Like Kinase 1 in Tomato Confers Resistance to Fusarium oxysporum f. sp. Radicis-Lycopersici
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WB
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Citation
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Ying Sun,Sheng-Wei Zhang
Mutual regulation of receptor-like kinase SIT1 and B'κ-PP2A shapes the early response of rice to salt stress.
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WB
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rice
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Citation
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