HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10] (ET1703-13)
Catalog# ET1703-13
Rabbit Monoclonal to GST
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WB
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Species independent
Lane 1: 293T transfected with GST cell lysate (5 µg/Lane)
Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane)
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-13) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10] (ET1703-13)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
293T transfected with GST cell lysate, 293T transfected with GST-tagged Histone H3.1 cell lysate.
Conjugation
Hrp
Clonality
Monoclonal
Clone Number
JM57-10
RRID
APPLICATION DILUTION
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WB
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1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
GST
SYNONYMS
Glutathione S Transferase antibody;Glutathione S-transferase class-mu 26 kDa isozyme antibody;GST antibody
FUNCTION
A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 kDa) in size,[40] which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. In addition to functioning as a purification tag, GST acts as a chaperone for the attached protein, promoting its correct folding, as well as preventing it from becoming aggregated in inclusion bodies when expressed in bacteria. The GST tag can easily be removed following purification by addition of thrombin protease if a suitable cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids).
CITATIONS
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The triglyceride catabolism regulated by a serine/threonine protein phosphatase, Smek1, is required for development and plant infection in Magnaporthe oryzae
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WB
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Rice
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Citation
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