GUCY1B3 Recombinant Rabbit Monoclonal Antibody [JE41-09] (HA722314)
Catalog# HA722314
Rabbit Monoclonal to GUCY1B3
Tested for
-
WB
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of GUCY1B3 on different lysates with Rabbit anti-GUCY1B3 antibody (HA722314) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate
Lane 2: Human brain tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse spleen tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722314) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Human lung tissue lysate
Lane 2: Human brain tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse spleen tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722314) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.