HDAC6 Recombinant Rabbit Monoclonal Antibody [JJ09-09] (ET1701-66)
Catalog# ET1701-66
Rabbit Monoclonal to HDAC6
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WB
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IF-Tissue
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IHC-P
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IP
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Human
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Monkey
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 160 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
HDAC6 Recombinant Rabbit Monoclonal Antibody [JJ09-09] (ET1701-66)
Immunogen
Synthetic peptide within human hdac6 aa 1-50 / 1,215.
Host
Rabbit
Positive Control
HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, COS-1 cell lysate, HCT 116 cell lysate, human kidney tissue, human breast tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ09-09
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:400
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 131 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
HDAC6
SYNONYMS
CPBHM antibody;FLJ16239 antibody;HD 6 antibody;HD6 antibody;HDAC 6 antibody;HDAC6 antibody;HDAC6_HUMAN antibody;Histone deacetylase 6 (HD6) antibody;Histone deacetylase 6 antibody;JM 21 antibody;JM21 antibody;KIAA0901 antibody;OTTHUMP00000032398 antibody;OTTHUMP00000197663 antibody;PPP1R90 antibody;Protein phosphatase 1 regulatory subunit 90 antibody
SEQUENCE SIMILARITIES
Belongs to the histone deacetylase family. HD type 2 subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by AURKA.; Ubiquitinated. Its polyubiquitination however does not lead to its degradation.; Sumoylated in vitro.
SUBCELLULAR LOCATION
Cell projection, Cytoplasm, Cytoskeleton, Nucleus.
FUNCTION
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.
CITATIONS
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HDAC6 Degrades nsp8 of Porcine Deltacoronavirus through Deacetylation and Ubiquitination to Inhibit Viral Replication
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WB
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Human
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Citation
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Dietary glucose increases glucose absorption and lipid deposition via SGLT1/2 signaling and acetylated ChREBP in the intestine and isolated intestinal epithelial cells of yellow catfish
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WB
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Yellow catfish
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Citation
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