HIF-2 alpha Recombinant Rabbit Monoclonal Antibody [JB24-42] (ET7107-32)

UNIPROT #

Q99814

PROTEIN NAME

HIF-2 alpha

SYNONYMS

Basic helix loop helix PAS protein MOP2 antibody;Basic-helix-loop-helix-PAS protein MOP2 antibody;bHLHe73 antibody;Class E basic helix-loop-helix protein 73 antibody;ECYT4 antibody;Endothelial PAS domain containing protein 1 antibody;Endothelial pas domain protein 1 antibody;Endothelial PAS domain-containing protein 1 antibody;EPAS 1 antibody;EPAS-1 antibody;EPAS1 antibody;EPAS1_HUMAN antibody;HIF 1 alpha like factor antibody;HIF 2 alpha antibody;HIF-1-alpha-like factor antibody;HIF-2-alpha antibody;HIF2-alpha antibody;HIF2A antibody;HLF antibody;Hypoxia inducible factor 2 alpha antibody;Hypoxia inducible factor 2 alpha subunit antibody;Hypoxia-inducible factor 2-alpha antibody;Member of PAS protein 2 antibody;Member of pas superfamily 2 antibody;MOP 2 antibody;MOP2 antibody;PAS domain-containing protein 2 antibody;PASD2 antibody

TISSUE SPECIFICITY

Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

POST-TRANSLATIONAL MODIFICATION

In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity).; In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.; Phosphorylated on multiple sites in the CTAD.; The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

SUBCELLULAR LOCATION

Nucleus, Nucleus speckle.

FUNCTION

Endothelial PAS domain-containing protein 1 (EPAS1, also known as hypoxia-inducible factor-2alpha (HIF-2α)) is a protein that is encoded by the EPAS1 gene in mammals. It is a type of hypoxia-inducible factor, a group of transcription factors involved in the physiological response to oxygen concentration. The gene is active under hypoxic conditions. It is also important in the development of the heart, and for maintaining the catecholamine balance required for protection of the heart. Mutation often leads to neuroendocrine tumors. The EPAS1 gene encodes one subunit of a transcription factor involved in the induction of genes regulated by oxygen, and which is induced as oxygen concentration falls (hypoxia). The protein contains a basic helix-loop-helix protein dimerization domain as well as a domain found in signal transduction proteins which respond to oxygen levels. EPAS1 is involved in the development of the embryonic heart and is expressed in endothelial cells that line the walls of blood vessels in the umbilical cord. EPAS1 is also essential for the maintenance of catecholamine homeostasis and protection against heart failure during early embryonic development. Catecholamines regulated by EPAS1 include epinephrine and norepinephrine. It is critical that the production of catecholamines remain in homeostatic conditions so that both the delicate fetal heart and the adult heart do not overexert themselves and induce heart failure. Catecholamine production in the embryo is related to control of cardiac output by increasing the fetal heart rate.