Histone H2B Rabbit Polyclonal Antibody (HA500381)
Catalog# HA500381
Rabbit Polyclonal to Histone H2B
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
-
Zebrafish
Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (HA500381) at 1/2,000 dilution.
Lane 1: MCF-7 cell lysate (10 µg/Lane)
Lane 2: Hela cell lysate (10 µg/Lane)
Lane 3: A549 cell lysate (10 µg/Lane)
Lane 4: 293T cell lysate (10 µg/Lane)
Lane 5: THP-1 cell lysate (10 µg/Lane)
Lane 6: K562 cell lysate (10 µg/Lane)
Lane 7: HL-60 cell lysate (10 µg/Lane)
Lane 8: NIH/3T3 cell lysate (10 µg/Lane)
Lane 9: Zebrafish tissue lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (10 µg/Lane)
Lane 11: RAW264.7 cell lysate (10 µg/Lane)
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500381) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF-7 cell lysate (10 µg/Lane)
Lane 2: Hela cell lysate (10 µg/Lane)
Lane 3: A549 cell lysate (10 µg/Lane)
Lane 4: 293T cell lysate (10 µg/Lane)
Lane 5: THP-1 cell lysate (10 µg/Lane)
Lane 6: K562 cell lysate (10 µg/Lane)
Lane 7: HL-60 cell lysate (10 µg/Lane)
Lane 8: NIH/3T3 cell lysate (10 µg/Lane)
Lane 9: Zebrafish tissue lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (10 µg/Lane)
Lane 11: RAW264.7 cell lysate (10 µg/Lane)
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500381) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.