HLA Class 1 ABC Mouse Monoclonal Antibody [11C2] (EM1801-10)
Catalog# EM1801-10
Mouse Monoclonal to HLA Class 1 ABC
-
WB
-
IHC-P
-
Human
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 2 minutes 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
HLA Class 1 ABC Mouse Monoclonal Antibody [11C2] (EM1801-10)
Immunogen
Recombinant protein within human hla class 1 b aa 1-362 / 362.
Host
Mouse
Positive Control
HeLa cell lysate, HepG2 cell lysate, 293T, human liver cancer tissue, human kidney tissue, mouse testis tissue, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
11C2
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
IHC-P:1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 41 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
HLA Class 1 ABC
SYNONYMS
Antigen presenting molecule antibody;HLA class I histocompatibility antigen, A 1 alpha chain antibody;HLAA antibody;Leukocyte antigen class I A antibody;Major histocompatibility complex, class I, A antibody;MHC class I antigen HLA A heavy chain antibody;AS antibody;HLA B protein antibody;HLA class I histocompatibility antigen, B alpha chain antibody;HLA-B antibody;HLA-B histocompatibility type antibody;HLAB antibody;leukocyte antigen class I-B antibody;lymphocyte antigen antibody;major histocompatibility complex, class I, B antibody;MHC class I antigen GN00104 antibody;MHC class I antigen HLA-B heavy chain antibody;MHC class I antigen SHCHA antibody;MHC Class I HLA heavy chain antibody;MHC HLA-B cell surface glycoprotein antibody;MHC HLA-B transmembrane glycoprotein antibody;SPDA1 antibody;1C07_HUMAN antibody;Cw-7 alpha chain antibody;D6S204 antibody;HLA class I histocompatibility antigen antibody;HLA class I histocompatibility antigen, C alpha chain antibody;HLA class I histocompatibility antigen, Cw-1 alpha chain antibody;HLA JY3 antibody;HLA-C antibody;HLA-C histocompatibility type antibody;HLA-Cw antibody;HLA-JY3 antibody;HLAC antibody;HLC C antibody;HLC-C antibody;human leukocyte antigen-C alpha chain antibody;major histocompatibility antigen HLA-C antibody;major histocompatibility complex, class I, C antibody;MHC class I antigen antibody;MHC class I antigen Cw 1 antibody;MHC class I antigen Cw*7 antibody;MHC class I antigen heavy chain HLA-C antibody;PSORS1 antibody
SUBCELLULAR LOCATION
Cell membrane, Endoplasmic reticulum membrane .
FUNCTION
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
