HMGB1 Recombinant Rabbit Monoclonal Antibody [SA39-03] (ET1601-2)

UNIPROT #

P09429

PROTEIN NAME

HMGB1

SYNONYMS

Amphoterin antibody;Chromosomal protein, nonhistone, HMG1 antibody;DKFZp686A04236 antibody;High mobility group 1 antibody;High mobility group box 1 antibody;High mobility group protein 1 antibody;High mobility group protein B1 antibody;high-mobility group (nonhistone chromosomal) protein 1 antibody;HMG-1 antibody;HMG1 antibody;HMG3 antibody;HMGB 1 antibody;HMGB1 antibody;HMGB1_HUMAN antibody;NONHISTONE CHROMOSOMAL PROTEIN HMG1 antibody;SBP 1 antibody;Sulfoglucuronyl carbohydrate binding protein antibody

SEQUENCE SIMILARITIES

Belongs to the HMGB family.

TISSUE SPECIFICITY

Ubiquituous. Expressed in platelets.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion.; Acetylated on multiple sites upon stimulation with LPS. Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).; Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).; Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).; In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance. Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity).

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cell membrane, Secreted, Chromosome

FUNCTION

High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. HMG-1 belongs to the high mobility group and contains a HMG-box domain. Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, it stays in the nucleus, but hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 has been shown to play an important role in helping the RAG endonuclease form a paired complex during V(D)J recombination.