HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] (HA601037)
Catalog# HA601037
Mouse Monoclonal to beta Actin
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WB
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Human
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Mouse
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Rat
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.
Specifications
Product Type
Recombinant Mouse monoclonal primary
Product Name
HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] (HA601037)
Immunogen
Synthetic peptide within n-terminal human beta-actin .
Host
Mouse
Positive Control
HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, THP-1 cell lysate, MCF-7 cell lysate, RH-35 cell lysate, L6 cell lysate, MEF cell lysate, HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, A549 cell lysate, PMVEC cell lysate.
Conjugation
Hrp
Clonality
Monoclonal
Clone Number
A2-F6-R
RRID
APPLICATION DILUTION
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WB
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1:2,500-1:20,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
Concentration
0.5ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
beta Actin
SYNONYMS
A26C1A antibody;A26C1B antibody;ACTB antibody;ACTB_HUMAN antibody;Actin beta antibody;Actin cytoplasmic 1 antibody;Actin, cytoplasmic 1, N-terminally processed antibody;Actx antibody;b actin antibody;Beta cytoskeletal actin antibody;Beta-actin antibody;BRWS1 antibody;E430023M04Rik antibody;MGC128179 antibody;PS1TP5 binding protein 1 antibody;PS1TP5BP1 antibody;β-actin antibody;β actin antibody
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
CITATIONS
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Zhang Haizheng,et al
Low-level Nd:YAG laser inhibiting inflammation and oxidative stress in human gingival fibroblasts via AMPK/SIRT3 axis
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WB
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Human
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Citation
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Li Kehan,et al
Hippo/YAP1 promotes osteoporotic mice bone defect repair via the activating of Wnt signaling pathway
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WB
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Mouse
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Citation
-
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Xiong Yi,et al
Blockade of the mitochondrial DNA release ameliorates hepatic ischemia-reperfusion injury through avoiding the activation of cGAS-Sting pathway
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WB
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Mouse
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Citation
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