HSC70 Interacting Protein HIP Recombinant Rabbit Monoclonal Antibody [JE33-17] (HA722274)
Catalog# HA722274
Rabbit Monoclonal to HSC70 Interacting Protein HIP
Tested for
-
WB
Reacts with
-
Human
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Mouse
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HSC70 Interacting Protein HIP on different lysates with Rabbit anti-HSC70 Interacting Protein HIP antibody (HA722274) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: A549 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 47 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: A549 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 47 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.