Hsp27 Recombinant Rabbit Monoclonal Antibody [JJ09-13] (ET1701-70)
Catalog# ET1701-70
Rabbit Monoclonal to Hsp27
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
FC
-
Human
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Mouse
-
Rat
Lane 1: HeLa cell lysate
Lane 2: MDA-MB-231 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-70) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Hsp27 Recombinant Rabbit Monoclonal Antibody [JJ09-13] (ET1701-70)
Immunogen
Synthetic peptide within human hsp27 aa 127-176 / 205.
Host
Rabbit
Positive Control
HeLa cell lysate, MDA-MB-231 cell lysate, MCF7 cell lysate, Mouse heart tissue lysate, HeLa, human colon cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ09-13
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
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IF-Cell
-
1:100-1:500
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IF-Tissue
-
1:100-1:500
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IHC-P
-
1:20,000
-
FC
-
1:1,000
-
IP
-
Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Hsp27
SYNONYMS
Heat shock 27kDa protein antibody;28 kDa heat shock protein antibody;CMT2F antibody;DKFZp586P1322 antibody;epididymis secretory protein Li 102 antibody;Estrogen regulated 24 kDa protein antibody;Estrogen-regulated 24 kDa protein antibody;Heat shock 25kDa protein 1 antibody;Heat shock 27 kDa protein antibody;Heat shock 27kD protein 1 antibody;Heat shock 27kDa protein 1 antibody;Heat shock 28kDa protein 1 antibody;Heat Shock Protein 27 antibody;Heat shock protein beta 1 antibody;Heat shock protein beta-1 antibody;heat shock protein family B (small) member 1 antibody;HEL-S-102 antibody;HMN2B antibody;HS.76067 antibody;Hsp 25 antibody;HSP 27 antibody;Hsp 28 antibody;Hsp B1 antibody;Hsp25 antibody;HSP27 antibody;Hsp28 antibody;HspB1 antibody;HSPB1_HUMAN antibody;SRP27 antibody;Stress responsive protein 27 antibody;Stress-responsive protein 27 antibody
SEQUENCE SIMILARITIES
Belongs to the small heat shock protein (HSP20) family.
TISSUE SPECIFICITY
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.
SUBCELLULAR LOCATION
Cytoplasm, Cytoskeleton, Nucleus.
FUNCTION
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27, HSP 32 and HSP 40. HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress induced by insult. Heat, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. In vitro, HSP 27 becomes highly phosphorylated following exposure to stress. The discovery that HSP 27 is regulated by hormones such as estrogen has led to studies establishing a relationship between HSP 27 and breast cancer.
