HSP60 Mouse Monoclonal Antibody [1-80] (EM00704)
Catalog# EM00704
Mouse Monoclonal to HSP60
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: SW480 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Rat kidney tissue lysate (no heat) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM00704) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
HSP60 Mouse Monoclonal Antibody [1-80] (EM00704)
Immunogen
Synthetic peptide within human hsp60 aa 524-573.
Host
Mouse
Positive Control
HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Rat kidney tissue lysate, HeLa, NIH/3T3, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
1-80
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:1,000
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IHC-P
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1:1,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 61 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
HSP60
SYNONYMS
60 kDa chaperonin antibody;60 kDa heat shock protein, mitochondrial antibody;CH60_HUMAN antibody;Chaperonin 60 antibody;Chaperonin, 60-KD antibody;CPN60 antibody;fa04a05 antibody;GROEL antibody;heat shock 60kDa protein 1 (chaperonin) antibody;Heat shock protein 1 (chaperonin) antibody;Heat shock protein 60 antibody;Heat shock protein 65 antibody;heat shock protein family D (Hsp60) member 1 antibody;HLD4 antibody;Hsp 60 antibody;HSP 65 antibody;HSP-60 antibody;HSP60 antibody;HSP65 antibody;HSPD1 antibody;HuCHA60 antibody;Mitochondrial matrix protein P1 antibody;P60 lymphocyte protein antibody;short heat shock protein 60 Hsp60s1 antibody;SPG14 antibody
SEQUENCE SIMILARITIES
Belongs to the chaperonin (HSP60) family.
SUBCELLULAR LOCATION
Mitochondrion matrix
FUNCTION
HSP60, also known as chaperonins (Cpn), is a family of heat shock proteins originally sorted by their 60kDa molecular mass. They prevent misfolding of proteins during stressful situations such as high heat, by assisting protein folding. HSP60 belong to a large class of molecules that assist protein folding, called molecular chaperones. Newly made proteins usually must fold from a linear chain of amino acids into a three-dimensional tertiary structure. The energy to fold proteins is supplied by non-covalent interactions between the amino acid side chains of each protein, and by solvent effects. Most proteins spontaneously fold into their most stable three-dimensional conformation, which is usually also their functional conformation, but occasionally proteins mis-fold. Molecular chaperones catalyze protein refolding by accelerating partial unfolding of misfolded proteins, aided by energy supplied by the hydrolysis of adenosine triphosphate (ATP). Chaperonin proteins may also tag misfolded proteins to be degraded.
CITATIONS
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HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-κB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function
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IF-Cell
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Human
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Citation
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