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Human Irisin enzyme-linked immunoassay kit(EH0081)

Price: Regular price $499.00

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Catalog# EH0081

Assay type Sandwich ELISA

Range 11.14-100.57

Sensitivity 2.60 pg/ml (50 μl)

PERFORMANCE CHARACTERISTICS

Product_detail

pg/ml O.D. Average Corrected
0.00 0.0272 0.0286 0.0279
15.63 0.0627 0.0626 0.0627 0.0348
31.25 0.1025 0.1015 0.1020 0.0741
62.50 0.2029 0.2004 0.2017 0.1738
125.00 0.4505 0.4487 0.4496 0.4217
250.00 1.1510 1.1050 1.1280 1.1001
500.00 2.6620 2.5750 2.6185 2.5906
1000.00 4.1606 4.1981 4.1794 4.1515

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)21.5 97.1 301.0 19.594.8305.4
Standard Deviation0.6 0.9 6.7 1.21.66.3
Coefficient of Variation(%)2.712.25.91.72.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3011.14-100.5710026.84

Sensitivity

2.60 pg/ml (50 μl)

Range

11.14-100.57

Recovery

The spike recovery was evaluated by spiking 3 levels of human Irisin into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 86% to 118% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Human Irisin enzyme-linked immunoassay kit(EH0081)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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