IDH2 Recombinant Rabbit Monoclonal Antibody [JA55-31] (ET1704-93)
Catalog# ET1704-93
Rabbit Monoclonal to IDH2
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF7 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: L6 cell lysate
Predicted band size: 51 kDa
Observed band size: 45 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-93) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
IDH2 Recombinant Rabbit Monoclonal Antibody [JA55-31] (ET1704-93)
Immunogen
Synthetic peptide within human idh2 aa 403-452 / 452.
Host
Rabbit
Positive Control
THP-1 cell lysate, Jurkat cell lysate, MCF7 cell lysate, C2C12 cell lysate, L6 cell lysate, human liver tissue, human tonsil tissue, human prostate tissue, rat heart tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA55-31
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:200
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 51 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
IDH2
SYNONYMS
D2HGA2 antibody;ICD-M antibody;IDH antibody;IDH2 antibody;IDHM antibody;IDHP_HUMAN antibody;IDP antibody;IDPM antibody;Isocitrate dehydrogenase [NADP], mitochondrial antibody;Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody;mNADP-IDH antibody;NADP(+)-specific ICDH antibody;Oxalosuccinate decarboxylase antibody
SEQUENCE SIMILARITIES
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
POST-TRANSLATIONAL MODIFICATION
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by the IDH2 gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Somatic mosaic mutations of this gene have also been found associated to Ollier disease and Maffucci syndrome.
CITATIONS
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Xie Jiaojiao,et al
Fibroblast growth factor 21 enhances learning and memory performance in mice by regulating hippocampal L-lactate homeostasis
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WB
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Mouse
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Citation
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